Effects of culture conditions on enhancement of 2,4-dinitrotoluene degradation by Burkholderia engineered with the Vitreoscilla hemoglobin gene

Citation
Ma. Nasr et al., Effects of culture conditions on enhancement of 2,4-dinitrotoluene degradation by Burkholderia engineered with the Vitreoscilla hemoglobin gene, BIOTECH PR, 17(2), 2001, pp. 359-361
Citations number
23
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY PROGRESS
ISSN journal
87567938 → ACNP
Volume
17
Issue
2
Year of publication
2001
Pages
359 - 361
Database
ISI
SICI code
8756-7938(200103/04)17:2<359:EOCCOE>2.0.ZU;2-R
Abstract
Growth and degradation of 2,4-dinitrotoluene (2,4-DNT) were compared in liq uid cultures in shake flasks for Burkholderia sp. strain DNT and strain DNT engineered to produce Vitreoscilla (bacterial) hemoglobin (strain Wi). Par ameters varied included aeration rate, initial 2,4-DNT concentration (50 an d 200 ppm!, and concentration and type of cosubstrate (yeast extract, succi nate, casamino acids, and tryptic soy broth). 2,4-DNT degradation increased with increasing cosubstrate concentration and was greater for strain WI th an strain DNT under most conditions tested; the greatest advantages of YV1 (up to 3.5-fold) occurred under limited aeration. A third strain (YV1m), de rived from YV1 by repeated growth on 2,4-DNT-containing medium, demonstrate d increased 2,4-DNT degradation (up to 1.3-fold compared to YV1) at 200 ppm 2,4-DNT. The growth profiles of the three strains with respect to each oth er were in general similar to those of the degradation patterns of 2,4-DNT.