Engineering the haem monooxygenase cytochrome P450(cam) for monoterpene oxidation

Monooxygenated terpenes are fine fragrance and flavouring chemicals, and ac tive site mutants of the haem monooxygenase cytochrome P450(cam) which were designed to have improved complementarity between the substrate binding po cket and the monoterpenes (+)-alpha -pinene (1) and S-limonene (2) have bee n shown to have greatly enhanced activity for the oxidation of these two su bstrates, and the major products, verbenol and isopiperitenol from (1) and (2) respectively, were formed with high regioselectivity and near-total ste reoselectivity.