S. Bystricky et al., Nonbiodegradable hyaluronan derivative prepared by reaction with a water-soluble carbodiimide, CHEM PAP-CH, 55(1), 2001, pp. 49-52
Reaction of hyaluronan (HA) with 1-ethyl-3-(3-dimethylaminopropyl)carbodiim
ide (EDC) yielded a water-soluble polysaccharide with 40 % of modified HA c
arboxyl groups. H-1 and C-13 NMR measurements revealed that hyaluronan cont
ained EDC bound by a nonspecific acylurea-type linkage. A test with testicu
lar hyaluronidase showed that the modified hyyaluronan was resistant to enz
ymic degradation.