P. Ellneskog-staam et al., Trigenomic origin of the hexaploid Psammopyrum athericum (Triticeae : Poaceae) revealed by in-situ hybridization, CHROMOS RES, 9(3), 2001, pp. 243-249
The genomic constitution of the hexaploid Psammopyrum athericum was studied
with in-situ DNA hybridization using both genomic DNA and isolated cloned
sequences as probes. A genomic probe from Thinopyrum bessarabicum (E genome
) hybridized successfully to 14 chromosomes of Ps. athericum and a probe fr
om Festucopsis serpentinii (L genome) hybridized to another 14 chromosomes.
The remaining chromosomes did not hybridize, apart from in the centromeric
region, to any of the genomic probes used. It is thus proposed that Ps. at
hericum contains the genomes E, L and X where X stands for a so-far unknown
genome. Psammopyrum athericum has three pairs of pTa71 sites and approxima
tely 30 pSc119:2 sites. The origin of the third genome will be a matter for
further research using genomic and genome-specific probes.