Modulation of soluble phases of endothelial/leukocyte adhesion molecule 1,intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 with interleukin-1 beta after experimental endotoxic challenge

Objective: To evaluate the effect of treatment with interleukin 1 beta (IL- 1 beta) on the concentrations of soluble adhesion molecules after an endoto xic challenge, Design:Randomized, controlled study. Setting: Experimental Unit, Virgen de las Nieves University Hospital. Subjects: Seventy-two female CBA/H mice of 20 to 21 g, supplied by the anim al center of the Experimental Unit. Intervention: The mice were randomized into three groups of 24, Group 1 (sh am) received two intraperitoneal tip) doses of 0.1 mt of phosphate-buffered saline; group 2 (lipopolysaccharide) was injected with 125 mg/kg lipopolys accharide (Escherichia coil) tip) 24 hrs after 0.1 mt of phosphate-buffered saline; group 3 was pretreated with 80 ng tip) of IL-1 beta per mouse 24 h rs before the endotoxic challenge. Measurements and Main Results:At 1, 2, 4, and 24 hrs after the endotoxic ch allenge, the concentrations of soluble endothelial/leukocyte adhesion molec ule 1 (ELAM-1), intercellular adhesion molecule 1 (ICAM-1), and vascular ce ll adhesion molecule 1 (VCAM-1) were measured in the three groups. There wa s a significant increase (p < .01) in these concentrations at these times i n comparison with the sham group, The use of IL-1<beta> produced a signific ant decrease (p < .05) in the three molecules among the treated group versu s the group submitted only to the challenge; concentrations of ELAM-1 signi ficantly decreased to below those of the sham group, and those of VCAM-1 re duced to levels that did not significantly differ from those of the sham gr oup. Conclusion: Endotoxin administration significantly increases the concentrat ions of soluble ELAM-1, ICAM-1, and VCAM-1 in mice, Treatment with IL-1<bet a> significantly decreases these concentrations, probably attenuating cell injury and organ dysfunction.