Peritoneal lavage with oxygenated perfluorochemical preserves intestinal mucosal barrier function after ischemia-reperfusion and ameliorates lung injury

Objective: To evaluate the effect of peritoneal lavage with an oxygenated p erfluorochemical (PFC) on intestinal ischemia-reperfusion injury (IIR), we assessed intestinal barrier function in terms of bacterial translocation an d endotoxemia, morphologic changes, and changes of intestinal luminal ph in rats subjected to lip, We also examined lung injury after IIR to test the effect of oxygenated PFC lavage on remote organ failure. Design:Prospective, randomized, and controlled animal study. Setting: Laboratory of a university hospital. Subject Male Sprague-Dawley rats. Interventions: Rats were subjected to ischemia by clipping the superior mes enteric artery. Reperfusion was achieved by release of the clip. Lavage of the abdominal cavity was performed by inflow and outflow of oxygenated PFC solution during ischemia. Results:Rats undergoing peritoneal lavage with oxygenated PFC (PFC group) s howed significantly better survival after IIR, The frequency of bacterial t ranslocation and the endotoxin concentration in superior mesenteric venous blood were significantly lower in the PFC group. Luminal acidosis also was alleviated in the PFC group, Furthermore, PFC lavage preserved the intestin al mucosal architecture and inhibited interstitial edema and infiltration o f inflammatory cells in the lungs. Conclusion:We conclude that peritoneal lavage with oxygenated PW: protects the intestinal mucosa and maintains mucosal barrier function after IIR, Pre servation of the intestinal mucosa ameliorates lung injury after IIR.