Mitochondrial ribosomal RNA in the germinal granules in Xenopus embryos revisited

Germ cells of various animals contain a determinant that is called the germ plasm. In amphibians such as Xenopus laevis, the germ plasm is composed of mitochondria and electron dense germinal granules that are embedded in a f ibrillar matrix, previous reports indicated that one of the components of g erminal granules was mitochondrial large and small ribosomal RNA (mtlrRNA a nd mtsrRNA). Utilizing a modified procedure for electron microscopy in situ hybridization, we investigated the distribution of these RNAs along with o ther components of the germ plasm in Xenopus laevis embryos. We found, that contrary to previous reports, the mtlrRNA and mtsrRNA were located in clos e vicinity to the germinal granules but were not major constituents of gran ules. The majority of the mtlrRNA and mtlsrRNAs was present inside the mito chondria and in the germ plasm matrix.