Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA

Citation
Dm. Macalpine et al., Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA, EMBO J, 20(7), 2001, pp. 1807-1817
Citations number
44
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO JOURNAL
ISSN journal
02614189 → ACNP
Volume
20
Issue
7
Year of publication
2001
Pages
1807 - 1817
Database
ISI
SICI code
0261-4189(20010402)20:7<1807:RAPIOH>2.0.ZU;2-V
Abstract
Wild-type yeast mitochondrial DNA (mtDNA) is inherited biparentally, wherea s mtDNA of hypersuppressive petite mutants is inherited uniparentally in cr osses to strains with wild-type mtDNA. Genomes of hypersuppressive petites contain a conserved ori sequence that includes a promoter, but it is unclea r whether the ori confers a segregation or replication advantage. Fluoresce nt in situ hybridization analysis of wild-type and petite mtDNAs in crosses reveals no preferential segregation of hypersuppressive petite mtDNA to fi rst zygotic buds. We identify single-stranded DNA circles and RNA-primed DN A replication intermediates in hypersuppressive petite mtDNA that are absen t from non-hypersuppressive petites. Mutating the promoter blocks hypersupp ressiveness in crosses to wild-type strains and eliminates the distinctive replication intermediates. We propose that promoter-dependent RNA-primed re plication accounts for the uniparental inheritance of hypersuppressive peti te mtDNA.