F1hD/F1hC-regulated promoters analyzed by gene array and lacZ gene fusions

The Escherichia coli transcriptional regulatory complex FlhD/FlhC, initiall y identified as a flagella-specific activator, is a global regulator involv ed in many cellular processes. Using gene arrays, lacZ gene fusions and enz yme assays, eight new targets of FlhD/FlhC were recognized. These are the t ransporter for galactose (MglBAC), the rod-shape determination proteins (Mr eBCD). malate dehydrogenase, and several enzymes involved in anaerobic resp iration (glycerol 3-phosphate dehydrogenase. GlpABC: periplasmic nitrate re ductase, NapFAGHBC: nitrite reductase, NrfABCDEFG: dimethyl sulfoxide reduc tase, DmsABC; and the modulator for hydrogenases, (C) 2001 Federation of Eu ropean Microbiological Societies. Published by Elsevier Science B.V. All ri ghts reserved.