Molecular profile of a human monoclonal antibody Fab fragment specific forEpstein-Barr virus gp350/220 antigen

Experimental evidence indicates Epstein Barr virus (EBV) envelope glycoprot ein gp350/220 elicits a potent virus neutralizing response in the infected human host that may play an important role in restricting viral pathogenesi s. In this study, we report the molecular cloning in combinatorial phage di splay vectors, of the IgG1 repertoire of an individual naturally infected w ith EBV, and describe the recovery and characterization of a monoclonal ant ibody recognizing gp350/220. A detailed understanding of the human antibody response in EBV infection will identify antibodies of potential use in ant iviral prophylaxis and will advance the production of more effective vaccin e candidates. (C) American Society for Histocompatibility and Immunogenetic s, 2001. Published by Elsevier Science Inc.