Ms. Pedro et al., Denaturing gradient gel electrophoresis analyses of microbial community from field-scale composter, J BIOSCI BI, 91(2), 2001, pp. 159-165
The diversity of microbial community during the decomposition of waste in a
held-scale composter (Hazaka system) was investigated by denaturing gradie
nt gel electrophoresis (DGGE). The composter operates at a high temperature
through a self-heating system, creating a thermophilic (60-76 degreesC) st
age during the initial phase acid a mesophilic (45 degreesC) stage towards
the later phase of the composting period. The pH of the system (pH 7.75-8.1
0) did not vary significantly during the process while moisture content was
reduced from 48.8% to 25.1%. DGGE and 16S rDNA analyses showed that the fo
llowing genera were found throughout the process: Propionibacterium sp., Me
thylobacterium sp., Pseudomonas sp., and Bradyrhizobium sp. Different Bacil
lus spp. thrive at the thermophilic or the mesophilic stage while Clostridi
um sp. was only found at the initial phase of the process. Staphylococcus s
p. and Caulobacter sp. or Brevundimonas sp. existed during the later phase
of the composting period.