Expedient liquid chromatographic method with fluorescence detection for montelukast sodium in micro-samples of plasma

This study describes an expedient assay for the analysis of the asthma medi cation, montelukast sodium (Singulair, MK-0476), in human plasma samples. A fter a simple extraction of the plasma, the drug and internal standard, qui nine bisulfate, were measured by HPLC. The chromatographic system consisted of a single pump, a refrigerated autosampler, a C-8 4-mum particle size ra dial compression cartridge at 40 degreesC and a fluorescence detector with the excitation and emission wavelengths set at 350 and 400 nm, respectively . The mobile phase which was delivered at 1.0 ml/min, was prepared by addin g 200 mi of 0.025 M sodium acetate, pH adjusted to 4.0 with acetic acid, to 800 ml of acetonitrile, with 50 mul triethylamine. With a run time of only 10 min per sample, this assay had an overall recovery of >97% with a detec tion limit of 1 ng/ml. The inter- and intra-run relative standard deviation s at 0.05, 0.2 and 1.0 mug/ml were all <9.2%, while the analytical recovery at the same concentrations were within 7.7% of the amount added. (C) 2001 Elsevier Science B.V. All rights reserved.