Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis

Citation
Aa. Hassan et al., Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis, J CLIN MICR, 39(4), 2001, pp. 1618-1621
Citations number
19
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
4
Year of publication
2001
Pages
1618 - 1621
Database
ISI
SICI code
0095-1137(200104)39:4<1618:EOPMFR>2.0.ZU;2-R
Abstract
Streptococcus uberis and Streptococcus parauberis reference strains and iso lates obtained from routine diagnostics were investigated by PCR,vith oligo nucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 is olates as S. uberis and 4 isolates as S. parauberis.