Random amplified polymorphic DNA assay as a rapid tool in screening for Neisseria meningitidis serogroup C isolates of electrophoretic type 24

Authors
Schmink, S Reeves, MW Plikaytis, B Popovic, T
Citation
S. Schmink et al., Random amplified polymorphic DNA assay as a rapid tool in screening for Neisseria meningitidis serogroup C isolates of electrophoretic type 24, J CLIN MICR, 39(4), 2001, pp. 1622-1625
Citations number
5
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
4
Year of publication
2001
Pages
1622 - 1625
Database
ISI
SICI code
0095-1137(200104)39:4<1622:RAPDAA>2.0.ZU;2-V
Abstract
Neisseria meningitidis serogroup C (NMSC) isolates of electrophoretic type 24 (ET-24), as identified by multilocus enzyme electrophoresis, are the mai n cause of serogroup C meningococcal disease outbreaks and sporadic meningo coccal disease in the United States. We evaluated a random amplified polymo rphic DNA assay as a rapid tool to screen for isolates of ET-24 by testing 199 NMSC isolates of 51 different ETs. A sensitivity of 88% and a specifici ty of 87% was achieved in identification of ET-24 isolates when the pattern s obtained by two primers, P1 and P5, were analyzed together.