Ovalbumin aerosols induce airway hyperreactivity in naive DO11.10 T cell receptor transgenic mice without pulmonary eosinophilia or OVA-specific antibody

Citation
Ja. Wilder et al., Ovalbumin aerosols induce airway hyperreactivity in naive DO11.10 T cell receptor transgenic mice without pulmonary eosinophilia or OVA-specific antibody, J LEUK BIOL, 69(4), 2001, pp. 538-547
Citations number
41
Categorie Soggetti
Immunology
Journal title
JOURNAL OF LEUKOCYTE BIOLOGY
ISSN journal
07415400 → ACNP
Volume
69
Issue
4
Year of publication
2001
Pages
538 - 547
Database
ISI
SICI code
0741-5400(200104)69:4<538:OAIAHI>2.0.ZU;2-Y
Abstract
The pathobiology of allergic asthma is being studied using murine models, m ost of which use systemic printing followed by pulmonary challenges with th e immunizing antigen. In general, mice develop eosinophilic pulmonary infla mmation, increased antigen-specific immunoglobulins, and airway hyperreacti vity (AHR), all of which are dependent on antigen-specific T cell activatio n. To establish a model of allergic asthma, which did not require systemic priming, we exposed DO11.10 T cell receptor transgenic mice, which have an expanded repertoire of ovalbumin (OVA), peptide-specific T cells, to limite d aerosols of OVA protein. DO11.10 +/- mice developed AHR in the absence of increases in total serum IgE, OVA-specific IgG, or eosinophilia, The AHR w as accompanied by pulmonary recruitment of antigen-specific T cells with de creased expression of CD62L and CD45RB and increased expression of CD69, a phenotype indicative of T cell activation. Our results support recent hypot heses that T cells mediate AHR directly.