Pregnancy after intentional cryopreservation of percutaneous sperm aspiration specimens and intracytoplasmic sperm injection - A report of two cases

BACKGROUND: Microsurgical epididymal sperm aspiration (MESA) and percutaneo us epididymal sperm aspiration (PESA) are two methods of obtaining spermato zoa from patients with irreparable obstructive azoospermia. Intentionally u sing frozen-thawed spermatozoa obtained from MESA had been reported to be s uccessful. With minimal invasiveness, intentionally cryopreserved PESA spec imens and intracytoplasmic sperm injection (ICSI) were carried out in two e ases. CASES: Two cases of irreparable obstructive azoospermia received PESA, and the spermatozoa were cryopreserved intentionally. Successful ICSI was perfo rmed later, utilizing frozen-thawed spermatozoa. CONCLUSION: PESA and ICSI are promising methods for these patients. The maj or advantages are minimal invasiveness and flexibility for further treatmen t.