Comparison of immediate intradermal test reactivity with serum IgE quantitation by use of a radioallergosorbent test and two ELISA in horses with andwithout atopy
G. Lorch et al., Comparison of immediate intradermal test reactivity with serum IgE quantitation by use of a radioallergosorbent test and two ELISA in horses with andwithout atopy, J AM VET ME, 218(8), 2001, pp. 1314-1322
Citations number
54
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
Objective-To compare a radioallergosorbent test and 2 ELISA with intraderma
l testing for the determination of environmental allergen hypersensitivity
in horses with and without atopic diseases.
Design-Prospective clinical study.
Animals-10 horses with recurrent urticaria, 7 with atopic dermatitis, 16 wi
th chronic obstructive pulmonary disease, and 22 without atopy.
Procedure-History, physical examination, hemogram, serum biochemical analys
es, bronchoalveolar ravage, and an intradermal test (used as the criterion
standard) with a regional panel of 73 allergens were performed in all horse
s. Serum was analyzed by use of the 3 in vitro assays of allergen-specific
IgE.
Results-An ELISA based on the a chain of the high-affinity IgE receptor, th
e Fee receptor immunoglobin E chain (Fc epsilon RI alpha) for IgE, had the
overall highest kappa statistic (0.238), positive predictive value (49%), a
nd negative predictive value (78%). Overall agreement between the Fc epsilo
n RI alpha -based ELISA and the intradermal test was fair. The highest kapp
a statistic was obtained by the Fc epsilon RI alpha -based ELISA in horses
with atopic dermatitis (0.330). Kappa statistics for the radioallergosorben
t test and a polyclonal antibody-based ELISA agreed slightly with that of t
he intradermal test at best.
Conclusions and Clinical Relevance-None of the 3 serum allergy tests reliab
ly detected allergen hypersensitivity, compared with the intradermal test.
The Fc epsilon RI alpha -based ELISA performed significantly better overall
than the other 2 tests. Low sensitivity of all 3 assays indicates the need
for continued study to elucidate a more sensitive test for the determinati
on of potentially pathogenic allergens in horses.