Objectives/Hypothesis: Up to the present, many reports have demonstrated th
at local immune response is associated with maintenance and persistence of
effusion in the middle ear cavity. Resulting retention of inflammatory cell
s and mediators in the middle ear results in ongoing effusion. The purpose
of this study was to clarify the role of tumor necrosis factor in experimen
tal otitis media with effusion, which was induced by transtympanic injectio
n of tumor necrosis factor in the rats.
Study Design: Four groups were designed in two experiments. The purpose of
experiment 1 was to confirm that transtympanic injection of TNF-alpha produ
ces the middle ear effusion In experiment 2, TNFsolRI was used to evaluate
the possibility as an inhibitor in otitis media with effusion.
Methods. The histopathological changes were observed under light microscope
, and the changes in microvascular permeability were examined using Evans b
lue vital dye technique.
Results: Middle ear effusion was developed in 70% of specimens, and histopa
thological changes, such as subepithelial edema and marked infiltration of
neutrophils, were present in 100% at 24 hours after administration of tumor
necrosis factor-alpha through transtympanic approach. Extravasation of Eva
ns blue dye was found in all specimens injected by tumor necrosis factor-al
pha which was qualified using a fluorescence microscope and quantified usin
g a spectrophotometer. These histopathological findings and changes in micr
ovascular permeability were significantly reduced by tumor necrosis factor
soluble receptor type I.
Conclusions: Neutrophil infiltration, subepithelial edema, increased microv
ascular permeability, and resultant effusion were indirectly proved to be i
nduced by tumor necrosis factor-alpha. We hope that this study may contribu
te to understanding the role of tumor necrosis factor-alpha in otitis media
with effusion and clarifying the future role of tumor necrosis factor solu
ble receptor type I in preventing otitis media with effusion.