Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion

Citation
Js. Hanna et al., Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion, MOL CELL B, 21(9), 2001, pp. 3144-3158
Citations number
120
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR AND CELLULAR BIOLOGY
ISSN journal
02707306 → ACNP
Volume
21
Issue
9
Year of publication
2001
Pages
3144 - 3158
Database
ISI
SICI code
0270-7306(200105)21:9<3144:SCCACA>2.0.ZU;2-A
Abstract
CTF4 and CTF18 are required for high-fidelity chromosome segregation. Both exhibit genetic and physical ties to replication fork constituents. We find that absence of either CTF4 or CTF18 causes sister chromatid cohesion fail ure and leads to a preanaphase accumulation of cells that depends on the sp indle assembly checkpoint. The physical and genetic interactions between CT F4, CTF18, and core components of replication fork complexes observed in th is study and others suggest that both gene products act in association with the replication fork to facilitate sister chromatid cohesion. We find that Ctf18p, an RFC1-like protein, directly interacts with Rfc2p, Rfc3p, Rfc4p, and Rfc5p. However, Ctf18p is not a component of biochemically purified pr oliferating cell nuclear antigen loading RF-C, suggesting the presence of a discrete complex containing Ctf18p, Rfc2p, Rfc3p, Rfc4p, and Rfc5p. Recent identification and characterization of the budding yeast polymerase kappa, encoded by TRF4, strongly supports a hypothesis that the DNA. replication machinery is required for proper sister chromatid cohesion. Analogous to th e polymerase switching role of the bacterial and human RF-C complexes, we p ropose that budding yeast RF-C-CTF18 may be involved in a polymerase switch event that facilities sister chromatid cohesion. The requirement for CTF4 and CTF18 in robust cohesion identifies novel roles for replication accesso ry proteins in this process.