Differences in sialic acid content of the hormone have been considered the
main determinant or FSH polymorphism. The aim of the present study was to i
nvestigate the effect of variations in the oligosaccharide structure of the
intrapituitary human FSH (hFSH) glycosylation variants on their intrinsic
biological activity. FSH charge isoforms obtained after chromatofocusing we
re further separated lay lectin affinity chromatography [Concanavalin A (Co
nA), Wheat germ agglutinin (WGA), Lentil lectin (LcH)]. Isolated isoforms w
ere separately tested for in-vitro bioactivity in a rat Sertoli cell aromat
ization bioassay. Our results show that: (1) FSH microheterogeneity is due
not only to variations in the sialic acid content of the hormone but also t
o differences in the internal structure of the carbohydrate chains, and (2)
variations in the sialic acid content as well as differences in the comple
xity of the glycans determine the full biological expression of FSH glycosy
lation variants. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved
.