Fibronectin fragment CH-296 inhibits apoptosis and enhances ex vivo gene transfer by murine retrovirus and human lentivirus vectors independent of viral tropism in nonhuman primate CD34(+) cells

Authors
Donahue, RE Sorrentino, BP Hawley, RG An, DS Chen, ISY Wersto, RP
Citation
Re. Donahue et al., Fibronectin fragment CH-296 inhibits apoptosis and enhances ex vivo gene transfer by murine retrovirus and human lentivirus vectors independent of viral tropism in nonhuman primate CD34(+) cells, MOL THER, 3(3), 2001, pp. 359-367
Citations number
57
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR THERAPY
ISSN journal
15250016 → ACNP
Volume
3
Issue
3
Year of publication
2001
Pages
359 - 367
Database
ISI
SICI code
1525-0016(200103)3:3<359:FFCIAA>2.0.ZU;2-5
Abstract
The fibronectin fragment CH-296 improved gene transfer to cytokine-mobilize d nonhuman primate CD34(+) cells irrespective of tropism to the MoMLV, GaLV , and VSV-C envelope proteins using murine stem cell virus (MSCV) and human immunodeficiency virus-1 (HIV-1)-based retrovirus vectors. For the HIV-1 l entivirus vector, CH-296 enhanced gene transfer in the absence of added hem atopoietic growth factors necessary for ex vivo stem cell expansion. In the presence of CH-296, apoptosis of CD34+ cells was inhibited, and in mobiliz ed peripheral blood CD34+ cells, cell division was stimulated as measured b y cell history/tracking experiments.