Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro

Citation
K. Yoshida et al., Mitochondrial genotypes and radiation-induced micronucleus formation in human osteosarcoma cells in vitro, ONCOL REP, 8(3), 2001, pp. 615-619
Citations number
20
Categorie Soggetti
Oncology
Journal title
ONCOLOGY REPORTS
ISSN journal
1021335X → ACNP
Volume
8
Issue
3
Year of publication
2001
Pages
615 - 619
Database
ISI
SICI code
1021-335X(200105/06)8:3<615:MGARMF>2.0.ZU;2-J
Abstract
We investigated whether or not the mitochondrial genotypes affect radiation -induced micronucleus (MN) formation. For that purpose, the rho (+), KT1 an d rho (0) human osteosarcoma cell lines were used, which carry the wild-typ e mitochondrial DNA (mtDNA), the tRNA(Lys) mutant mtDNA and no mtDNA, respe ctively. Despite no significant difference in the clonogenic radiosensitivi ty, the rho (+), KT1 and rho (0) cells exhibited high, intermediate and low radiosensitivities, respectively, to the MN induction in cytokinesis-block ed binucleated cells. Such differential MN inductions were correlated with high, intermediate and low levels of cellular ATP in the rho (+), KT1 and r ho (0) cells, respectively, but not exactly with ROS production. Antimycin A that inhibits the respiratory complex III reduced the rate of radiation-i nduced MN induction in the rho (+) and KT1, but not rho (0) cells. Thus, th e functional status of the mtDNA to produce ATP appears to play a significa nt role for radiation-induced MN.