Recovering DNA and optimizing PCR conditions from microdissected formalin-fixed and paraffin-embedded materials

Citation
Zp. Ren et al., Recovering DNA and optimizing PCR conditions from microdissected formalin-fixed and paraffin-embedded materials, PATHOBIOLOG, 68(4-5), 2000, pp. 215-217
Citations number
14
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
PATHOBIOLOGY
ISSN journal
10152008 → ACNP
Volume
68
Issue
4-5
Year of publication
2000
Pages
215 - 217
Database
ISI
SICI code
1015-2008(2000)68:4-5<215:RDAOPC>2.0.ZU;2-3
Abstract
Microdissection is a powerful technique in molecular pathology and genetic investigations, To detect genetic alterations such as gene mutation or dele tion from tumor specimen, the purity of target cells is extremely critical. Unwanted cell contamination will dramatically dilute the detectable level of the abnormality. The major obstacle in clinical research is to obtain su fficient and qualified DNA from a small amount of formalin-fixed and paraff in-embedded materials. We have successfully modified our previous protocols and overcome the difficulties of recovery of DNA, After these modification s, almost every single formalin-fixed and paraffin-embedded specimen has be en successfully amplified in the required DNA region. Copyright (C) 2001 S. Karger AG, Basel.