CONTRIBUTION OF IL-1-BETA TO THE ENHANCEMENT OF CAMPYLOBACTER-RECTUS LIPOPOLYSACCHARIDE-STIMULATED PGE(2) PRODUCTION IN OLD GINGIVAL FIBROBLASTS IN-VITRO

Campylobacter rectus is associated with adult periodontitis. We previo usly reported that C. rectus lipopolysaccharide (LPS)-stimulated prost aglandin E-2 (PGE(2)) production in old cells of human gingival fibrob lasts (HGFs) is higher than that in young cells. The present study exa mined whether an enhancement of C. rectus LPS-stimulated interleukin ( IL)-1 beta production in old HGFs contributed to the increased product ion of PGE(2). LPS was prepared from C. rectus ATCC33238. HGFs were es tablished from healthy gingiva in three patients, aged 10-12 years. Ce llular aging in culture was determined with increasing doubling. The c ultured cells were treated with LPS (0.01-10 mu g/ml), and the amount of IL-1 beta in the medium was measured after a 24 h incubation. The L PS-stimulated IL-1 beta production in each old cell (corresponding to 57-67% of complete life-span) was increased (1.6-2.6 times) compared t o that in the young cells (corresponding to 17-20% of the life-span). The IL-1 beta mRNA synthesis in the presence of LPS in the old cells w as higher than that in the young cells. The enhancement of LPS-stimula ted PGE(2) production was inhibited by anti-IL-1 beta antibody and by IL-I receptor antagonist. These findings suggest that the greater abil ity of old cells to produce PGE(2) in response to C. rectus LPS is due to their greater level of IL-1 beta. (C) 1997 Elsevier Science Irelan d Ltd.