Radiation sensitivity of lymphocytes from healthy individuals and cancer patients as measured by the comet assay

Citation
Wu. Muller et al., Radiation sensitivity of lymphocytes from healthy individuals and cancer patients as measured by the comet assay, RADIAT ENV, 40(1), 2001, pp. 83-89
Citations number
18
Categorie Soggetti
Multidisciplinary
Journal title
RADIATION AND ENVIRONMENTAL BIOPHYSICS
ISSN journal
0301634X → ACNP
Volume
40
Issue
1
Year of publication
2001
Pages
83 - 89
Database
ISI
SICI code
0301-634X(200103)40:1<83:RSOLFH>2.0.ZU;2-3
Abstract
Lymphocytes of healthy volunteers (n=24) and of tumour patients (n=30, 18 o f whom had experienced severe side-effects) were irradiated with x-rays in vitro. DNA damage was analysed after 0.25-2 Gy and DNA repair after 2 Gy, a nd quantification of both endpoints was done by the comet assay. The indivi dual differences in radiation-induced DNA damage as well as in the repair k inetics were observed to be striking for both healthy donors and tumour pat ients. After a repair time of 3 h. following 2 Gy x-irradiation, some of th e healthy volunteers showed no residual DNA damage at all in their lymphocy tes, whereas others revealed about 30%. There was no indication that our re sults were affected by either age, gender or smoking habits. Slow repair ki netics and high amounts of residual damage were characteristic for many but not all tumour patients who had experienced severe side-effects in their n ormal tissues during or after radiotherapy (n=18). Our conclusion is that t hose individuals showing poor DNA repair characteristics in the lymphocytes following in vitro irradiation, have a high probability of being radiosens itive. The opposite conclusion is not necessarily true: if repair is effect ive, this does not mean that the individual is radioresistant, because fact ors other than impaired repair may cause radiosensitivity.