Variability of storage proteins in some Czech landraces, modern and obsolete cultivars of wheat (Triticum aestivum L.)

A set of Czech landraces and obsolete cultivars was evaluated with respect to the allelic variations at Cli-l, Glu-l and Glu-3 loci. PCR based techniq ue was used for LMW-GSs evaluation. The method was proved to be suitable fo r study of wheat germplasm with respect to LMW-GSs allelomorphism. A higher diversity was found in the landraces and obsolete cultivars as compared wi th modern ones. Especially short amplification products corresponding to LM W-GSs with shorter central repetitive domains are missing in modern cultiva rs. Landraces and obsolete cultivars originating in the Czech Republic were proved to be a source of important variability with respect to LMW-GSs and could be used as a source of alternative alleles. Gliadin and HMW-GS allel omorphism evaluated on the protein level were similar to those found in com parable sets of wheat genotypes. No significant relationships were discover ed between gliadins and LMW-GS patterns based on clustering, even when Gli- l and Glu-3 loci are localised close to each other on the same chromosome a rms.