Use of amino-carbonyl reaction and chemiluminescence detection to the flow-injection determination of some amino acids

Some amino acids were found to react with carbonyl functional groups of hum ic acid. Products of this reaction give strong chemiluminescence during the ir oxidation with N-bromosuccinimide (NBS) in alkaline solution. Humic acid s from different sources produced similiar signal magnitude. This effect wa s employed to the flow-injection determination of glycine and arginine in p harmaceutical formulations with considerable selectivity against different amino compounds. The proposed method is fast and simple. Detection limit is 0.20 and 0.25 mg.l(-1) for glycine and arginine respectively, and 115 samp les per h can be determined. (C) 2001 Elsevier Science B.V. All rights rese rved.