Synthesis of the extracellular Ig domain I of Emmprin carrying a chitobiose unit

Authors
Hojo, H Watabe, J Nakahara, Y Nakahara, Y Ito, Y Nabeshima, K Toole, BP
Citation
H. Hojo et al., Synthesis of the extracellular Ig domain I of Emmprin carrying a chitobiose unit, TETRAHEDR L, 42(16), 2001, pp. 3001-3004
Citations number
8
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
42
Issue
16
Year of publication
2001
Pages
3001 - 3004
Database
ISI
SICI code
0040-4039(20010416)42:16<3001:SOTEID>2.0.ZU;2-I
Abstract
The extracellular Ig domain I of Emmprin (34-94) carrying a chitobiose unit at Asn(44) was chemically synthesized. Boc-Asn with a benzyl-protected chi tobiose unit was synthesized and used for the preparation of peptide thioes ter with the sequence of Emmprin (34-58) by Boc strategy. C-Terminal peptid e amide (59-94) was also prepared by the solid-phase method. These segments were condensed by activation of the thioester group by silver ions to obta in a protected form of Emmprin (34-94)-NH2. After deprotection and air oxid ation, the desired Emmprin (34-94)-NH2 with chitobiose was successfully obt ained. (C) 2001 Elsevier Science Ltd. All rights reserved.