Improved xylanase production from a haloalkalophilic Staphylococcus sp SG-13 using inexpensive agricultural residues

The production of an alkali-stable xylanase, with dual pH optima, from halo alkalophilic Staphylococcus sp. SG-13 has been enhanced using agro-residues in submerged fermentation and a biphasic growth system. The agro-residues such as wheat bran, sugarcane bagasse, corncobs and poplar wood when used a s sole carbon source, improved the xylanase yield by five-fold as compared to xylose and xylan. Staphylococcus sp. SG-13 also produced equally good am ounts of xylanase when grown simply in deionized water (pH 8.0) supplemente d with agro-residues as sole carbon source. In the biphasic growth system ( lower layer containing agricultural residue set in agar medium with liquid medium above it), the prime substrate, wheat bran (1% w/v), resulted in max imum xylanase production of 4525 U l(-1) (pH 7.5) and 4540 U l(-1) (pH 9.2) at an agar: broth ratio of 4.0 after 48 h of incubation at 37 degreesC und er static conditions. In general, the cost-effective agro-residues were fou nd to be more suitable inducers for xylanase production over expensive subs trates like xylan.