Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae MNN9 gene

A gene homologous to Saccharomyces cerevisiae MNN9 has been cloned and char acterized in the methylotrophic yeast Hansenula polymorpha. This gene was c loned from a H. polymorpha genomic DNA library using the S. cerevisiae MNN9 gene as a probe. The H. polymorpha MNN9 homologue (HpMNN9) contained a 106 2 bp open reading frame encoding a predicted protein of 354 amino acids, Th e deduced amino acid sequence showed 58% and 51% identity, respectively, wi th the S, cerevisiae and Candida albicans Mnn9 proteins. Disruption of HpMN N9 leads to phenotypic effects suggestive of cell wall defects, including d etergent sensitivity and hygromycin B sensitivity. The hygromycin B sensiti vity of S, cerevisiae mnn9 null mutant was complemented in the presence of the HpMNN9 gene. The DNA sequence of the H. polymorpha homologue has been s ubmitted to GenBank with the Accession No. AF264786. Copyright (C) 2001 Joh n Wiley & Sons, Ltd.