Mm. Sedensky et al., Model organisms: New insights into ion channel and transporter function. Stomatin homologues interact in Caenorhabditis elegans, AM J P-CELL, 280(5), 2001, pp. C1340-C1348
In C. elegans the protein UNC-1 is a major determinant of anesthetic sensit
ivity and is a close homologue of the mammalian protein stomatin. In humans
stomatin is missing from erythrocyte membranes in the hemolytic disease ov
erhydrated hereditary stomatocytosis, despite an apparently normal stomatin
gene. Overhydrated hereditary stomatocytosis is characterized by alteratio
n of the normal transmembrane gradients of sodium and potassium. Stomatin h
as been shown to interact genetically with sodium channels. It is also post
ulated that stomatin is important in the organization of lipid rafts. We de
monstrate here that antibodies against UNC-1 stain the major nerve tracts o
f Caenorhabditis elegans, with very intense staining of the nerve ring. We
also found that a gene encoding a stomatin-like protein, UNC-24, affects an
esthetic sensitivity and is genetically epistatic to unc-1. In the absence
of UNC-24, the staining of the nerve ring by anti-UNC-1 is abolished, despi
te normal transcriptional levels of the unc-1 mRNA. Western blots indicate
that UNC-24 probably affects the stability of the UNC-1 protein. UNC-24 may
therefore be necessary for the correct placement of UNC- 1 in the cell mem
brane and organization of lipid rafts.