Tg. Moreels et al., Effect of Schistosoma mansoni-induced granulomatous inflammation on murinegastrointestinal motility, AM J P-GAST, 280(5), 2001, pp. G1030-G1042
Citations number
44
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
In Schistosoma mansoni-infected mice, gastrointestinal transit was measured
in vivo and the neuromuscular function of longitudinal muscle strips of in
flamed ileum and noninflamed gastric fundus was assessed in vitro. Eight we
eks after infection, the ileal wall was acutely inflamed, as shown by a muc
osal inflammatory infiltrate, leading to an increase in mucosal thickness,
in myeloperoxidase (MPO) activity, and in interleukin (IL)-1 beta productio
n. At that time, both gastrointestinal transit and in vitro ileal contracti
lity were normal. Twelve weeks after infection, chronic granulomatous infla
mmation led to proliferation of the muscle layer and to a further increase
in MPO activity, whereas IL-1 beta production normalized. Gastrointestinal
transit was decreased, whereas in vitro ileal contractility was increased i
rrespective of the contractile stimulus. In vitro incubation with IL-1 beta
(10 ng/ml for 60 min) significantly increased ileal contractility only at
8 wk after infection. Indomethacin, tetrodotoxin, and atropine had no diffe
rential effect on ileal contractility in controls and infected mice. In vit
ro contractility of noninflamed gastric fundus was normal both 8 and 12 wk
after infection. We conclude that intestinal schistosomiasis 8 wk after inf
ection is associated only with structural changes of the ileum, whereas 12
wk after infection, both structural and functional changes are present. The
se changes are characterized by increased ileal wall thickness, decreased g
astrointestinal transit, and increased smooth muscle contractility restrict
ed to the inflamed gut segment.