Study of the spectrophotometric analysis of protein solution with p-iodochlorophosphonazo as adsorbate by the microphase adsorption-spectral correction technique

A microphase adsorption-spectral correction (MPASC) technique was establish ed, which consists of Langmuir adsorption and the spectral correction techn ique. The hypothesis of a microelectric field formed in macromolecules is p roposed, the existence of which results in the adsorption and aggregation o f dye molecules in macromolecules. This paper describes the spectrophotomet ric analysis of protein solution with p-iodochlorophosphonazo (p-ICPA) dye. The results showed that the adsorption ratios of p-ICPA to proteins (bovin e serum albumin, ovalbumin, myoglobin and gamma -globulin) at pH 2.56 were 54, 25, 14 and 90, respectively, their adsorption constants 2.86 X 10(5), 1 .42 X 10(6), 2.38 X 10(5) and 1.09 x 10(5) and their absorptivities 8.00 x 10(5), 4.02 X 10(5), 1.77 X 10(5) and 1.15 x 10(6) l mol(-1) cm(-1) at 620 nm. The analysis of practical samples showed that the recovery of protein w as between 92.3 and 108% and the relative standard deviation was 4.7%.