PECTINOLYTIC ENZYMES FROM PSEUDOMONAS-MARGINALIS MAFF-03-01173

Two pectinolytic enzymes were purified from the culture broth of Pseud omonas marginalis pv. marginalis MAFF 03-01173 with total 33% recovery of the initial activity. From the substrate specificities against pec tin and polygalacturonic acid, the requirement of calcium ion for the enzymatic activity, and the N-terminal sequences, the enzymes were ide ntified as pectin lyase and pectate lyase. The M(r)s of pectin lyase a nd pectate lyase were estimated to be 34000 and 43000, respectively, b y SDS polyacrylamide gel electrophoresis. Both enzymes showed almost t he same pH dependent activity curves with the highest activity at pH 8 .3. (C) 1997 Elsevier Science Ltd. All rights reserved.