Determination of substrate and product concentrations in lactic acid bacterial fermentations by proton NMR using the ERETIC method

Citation
V. Silvestre et al., Determination of substrate and product concentrations in lactic acid bacterial fermentations by proton NMR using the ERETIC method, ANALYT CHEM, 73(8), 2001, pp. 1862-1868
Citations number
26
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
00032700 → ACNP
Volume
73
Issue
8
Year of publication
2001
Pages
1862 - 1868
Database
ISI
SICI code
0003-2700(20010415)73:8<1862:DOSAPC>2.0.ZU;2-2
Abstract
A H-1 NMR method is described for the determination of the concentration of a chemically diverse group of metabolites over a wide concentration range in fermentation medium. The method employs the use of the ERETIC signal, wh ich produces a pseudo-FID acquired with the FID derived from the sample com ponents. It is shown that this signal, once calibrated against an external standard solution, can be used to determine accurately the concentration of all components present in the medium. The method is rapid, reliable, and a pplicable to a wide range of biological fluids. Problems such as the invisi bility of certain components in the spectrum are overcome by a simple regul ation of the pH.