Further considerations of the skeletal system as a biomarker of episodic chlorpyrifos exposure

The mummichog, Fundulus heteroclitus, is a common inhabitant of eastern sea board estuaries. As such, it can be affected by coastal agricultural and ot her nonpoint source runoff. We examined the effects of short-term episodic exposures to an agricultural pesticide, chlorpyrifos, on brain acetylcholin esterase (AChE) activity and vertebral yield strength in lab-reared and wil d-caught fish. Brain AChE activity was chosen as an indicator because it is the target system for organophosphate action. Vertebral yield strength was chosen as an indicator because previous research warranted further investi gation (Karen et al., 1998). Four daily or weekly 6 h exposures (2.5, 5.0, and 10.0 mug/l chlorpyrifos) in decreased salinity seawater (5 g/kg) signif icantly reduced brain AChE activity. The lowest concentration was within th e range of reported environmental chlorpyrifos concentrations; thus inhibit ion of brain AChE from environmental chlorpyrifos exposures may pose a haza rd to estuarine organisms. Yield strength measured in lab-reared fish appea red to be more sensitive to episodic chlorpyrifos exposures, because chlorp yrifos was a significant factor in 75% (3 out of 4) of tests performed with lab-reared fish. Chlorpyrifos exposure was a significant factor in only 25 % (1 out of 4) tests performed with wild fish. These results suggested that changes in the responses of bone to load testing, following several short exposures to an organophosphate, could be sensitive indicators in lab-reare d organisms. (C) 2001 Elsevier Science B.V. All rights reserved.