Genotyping of group A rotavirus samples from Brazilian children by probe hybridization

Authors
Cardoso, DDP Racz, ML Azevedo, MSP Martins, RMB Soares, CMA
Citation
Ddp. Cardoso et al., Genotyping of group A rotavirus samples from Brazilian children by probe hybridization, BRAZ J MED, 34(4), 2001, pp. 471-473
Citations number
19
Categorie Soggetti
Medical Research General Topics
Journal title
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
ISSN journal
0100879X → ACNP
Volume
34
Issue
4
Year of publication
2001
Pages
471 - 473
Database
ISI
SICI code
0100-879X(200104)34:4<471:GOGARS>2.0.ZU;2-#
Abstract
The G genotyping of 74 group A rotavirus samples was dune by RNA-DNA hybrid ization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 3. Thirty-one samples c ould be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4, and th e predominance of G1. The knowledge of genotypes provides important informa tion concerning rotavirus circulation in Central Brazil.