Determination of protein-protein interactions of ICln by the yeast two-hybrid system

ICIn is a ubiquitously expressed eukaryotic protein. Expression of the prot ein in Xenopus laevis oocytes, the knocking-down of the protein in fibrobla sts, or the reconstitution of the protein in lipid bilayer led to the assum ption that this protein is an ionic channel or a significant part thereof. However, other possible roles for ICIn in potential regulatory mechanisms h ave been postulated, as diverse as regulator of cell morphology by interact ing with the Skb1 protein and/or interaction with core spliceosomal protein s. Here we show that ICIn is able to interact with SnRNP core proteins SmD1 , SmD2, SmD3, SmX5 and SmB/B'. Copyright (C) 2001 S. Karger AG. Basel.