Five common biocatalytic process platforms for the production of enantiomer
ically pure amino acids are compared along four different dimensions of mer
it: i) enantioselectivity, ii) overall yield, iii) biocatalyst operating st
ability, and iv) reactor space-time yield. All processes practiced on indus
trial scale utilize biocatalysts of very high enantioselectivity. Short eff
icient process routes are a necessity, giving an inherent advantage to rout
es based on lyase reactions or reduction of prochiral keto acids. For proce
sses based on the splitting of a racemate, recycling the unwanted enantiome
r is crucial. The pKa value of abstracting the alpha -proton serves as a fi
rst indication of the difficulty of achieving overall yields far in excess
of 50% through dynamic resolution. High solubility of substrates and produc
ts was found to favor high reactor productivity (space-time-yield s.t.y. >
1 kg/(1.d)) and high biocatalyst productivity (enzyme consumption number e.
c.n. < 500-1000 U/kg of product).