Synthesis and fixation of aminocyanines to microcrystalline cellulose using cyanuric chloride as a cross-linking agent

Although monomethine cyanines and reactive dyes, used mainly for dyeing tex tile goods, are well studied, the development of cyanines with groups capab le of being bound covalently to a solid support has been largely unexplored . The scope of this study was the synthesis and characterisation of aminocy anines capable of binding to microcrystalline cellulose, through the polyfu nctional cross-linking agent cyanuric chloride. In a second approach, the c yanines bound to microcrystalline cellulose in varying amounts were used as specific ligands in affinity chromatography. Two different methods to dete rmine the amount or cyanine linked to the dyed cellulose were used, based o n Fourier transform infrared spectroscopy ansi elemental analysis. The fixa tion yields of the two different cyanines bound to cellulose were determine d.