Halenaquinol, a natural cardioactive pentacyclic hydroquinone, interacts with sulfhydryls on rat brain Na+,K+-ATPase

Halenaquinol inhibited the partial reactions of ATP hydrolysis by rat brain cortex Na+,K+-ATPase, such as [H-3]ATP binding to the enzyme. Na+-dependen t front-door phosphorylation from [gamma-P-33]ATP, and also Na+- and K+-dep endent E-1 <----> E-2 conformational transitions of the enzyme. Halenaquino l abolished the positive cooperativity between the Na+- and K+-binding site s on the enzyme. ATP and sulfhydryl-containing reagents (cysteine and dithi othreitol) protected the Na+,K+-ATPase against inhibition, Halenaquinol can react with additional vital groups in the enzyme after blockage of certain sulfhydryl groups with 5,5'-dithio-bis-nitrobenzoic acid. Halenaquinol inh ibited [H-3]ouabain binding to Na+,K+-ATPase under phosphorylating and non- phosphorylating conditions. Binding of fluorescein 5'-isothiocyanate to Na,K+-ATPase and intensity of fluorescence of enzyme tryptophanyl residues we re decreased by halenaquinol. We suggest that interaction of halenaquinol w ith the essential sulfhydryls in/or near the ATP-binding site of Na+,K+-ATP ase resulted in a change of protein conformation and subsequent alteration of overall and partial enzymatic reactions, (C) 2001 Elsevier Science Inc. All rights reserved.