Improved biocatalysts by directed evolution and rational protein design

Authors
Bornscheuer, UT Pohl, M
Citation
Ut. Bornscheuer et M. Pohl, Improved biocatalysts by directed evolution and rational protein design, CURR OP C B, 5(2), 2001, pp. 137-143
Citations number
64
Categorie Soggetti
Biochemistry & Biophysics
Journal title
CURRENT OPINION IN CHEMICAL BIOLOGY
ISSN journal
13675931 → ACNP
Volume
5
Issue
2
Year of publication
2001
Pages
137 - 143
Database
ISI
SICI code
1367-5931(200104)5:2<137:IBBDEA>2.0.ZU;2-3
Abstract
The efficient application of biocatalysts requires the; availability of sui table: enzymes with high activity and stability under process conditions, d esired substrate selectivity and high enantioselectivity. However, wild-typ e enzymes: often need to be optimized La fulfill these requirements. Two: r ather contradictory tools can be used on a, molecular level to create tailo r-made bio-catalysts: directed evolution and rational protein design.