Impact of Celsior solution on hepatocellular, reperfusion and endothelial cell injury after liver transplantation

Background Initially, preservation solutions were developed to maintain cel l function of the transplanted organs. However: recently developed preserva tion solutions also contain a variety of substances to reduce the reperfusi on injury. Aim, To study the effect of three different preservation solutions on the l iver cell injury, endothelial cell function and reperfusion injury after li ver transplantation. Material and Methods. Large White X Landrace pigs of either sex were subjec ted to orthotopic liver transplantation. Donor livers were flushed and stor ed in University of Wisconsin Solution, Eurocollins Solution or Celsior Sol ution for 3 hours. Blood samples were taken at various times post transplan tation for assessment of aspartate aminotransferase, hyaluronic acid, malon dialdehyde and Vitamin A levels. Results. Serum aspartate aminotransferase levels were lower in the livers p reserved in the Wisconsin solution. Plasma malondialdehyde levels were lowe r and serum Vitamin A levels were higher in the livers preserved in Celsior solution. Serum hyaluronic acid levels increased after liver transplantati on but were similar with all three solutions. Conclusions. There was less hepato-cellular injury in the livers preserved in Wisconsin solution and less reperfusion injury with the Celsior solution . The endothelial cell injury was similar with all three solutions.