Stable-carbon-isotope ratios of river biota: Implications for energy flow in lotic food webs

Stable-isotope ratios of carbon (C-13/C-12 or delta C-13) have been widely used to determine the energy base of stream food webs, but such use is cont roversial due to unexplained variability in algal delta C-13. I used publis hed delta C-13 data from temperate headwater streams through medium-sized r ivers (0.2-4000 km(2) watershed area) collected during summer baseflows and original data from streams in northern California to analyze energy pathwa ys through river food webs. The analyses showed three important results. Fi rst, epilithic algal delta C-13 and watershed area are positively related, suggesting that effects of carbon Limitation on algal carbon uptake result in C-13 enrichment of algal delta C-13 in larger, more productive rivers. S econd, epilithic algae and terrestrial detritus delta C-13 values are often distinct in small shaded streams but overlap in some larger unshaded strea ms and rivers. Measurements of delta C-13 values may be most useful in dist inguishing algal and terrestrial energy sources in unproductive streams wit h supersaturated dissolved CO, concentrations, and some productive rivers w here CO, concentrations are low relative to photosynthetic rates. Finally, consumer delta C-13 values are more strongly related to algal delta C-13 th an terrestrial delta C-13. The relative contribution of terrestrial and alg al carbon sources often varied by functional feeding group within and betwe en sites. However, with the exception of shredders and scrapers, which resp ectively relied on terrestrial and algal carbon sources, patterns of consum er delta C-13 clearly show a transition from terrestrial to algal carbon so urces for many lotic food webs in streams with greater than or equal to 10 km(2) watershed area. The observed transition to algal carbon sources is li kely related to increasing primary production rates as forest canopy cover declines in larger streams, although decreasing retention or quality of ter restrial carbon may also play a role. Improved analyses of algal delta C-13 and delta N-15 combined with quantitative study of organic matter dynamics and food web structure should allow the relative importance of these facto rs to be distinguished in future food web studies.