The optimum of a species on a gradient is an important parameter for ecolog
ical interpretation and bioindication. The location of the optimum is easil
y estimated in the popular Gaussian response model, but it is more difficul
t to assess the precision of the estimated optima. Methods based on the pro
file likelihood or quasilikelihood function are presented to find confidenc
e intervals for the optimum parameter of the Gaussian response function usi
ng generalized linear models. The following four cases are considered: opti
mum on one gradient; optimum on one gradient when there are additional stra
tifying variables; optimum on an interesting gradient at a certain level of
a stratifying variable when the optimum is dependent on the latter; and si
multaneous confidence region for the joint overall optimum on two gradients
. The methods are illustrated with two species of testate amoebae (Protozoa
: Rhizopoda) in Finnish mires. The first two cases were also analyzed using
Fieller's theorem, although it produced generally wider limits.