Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis

Authors
Sutherland, JC Monteleone, DC Trunk, JG Bennett, PV Sutherland, BM
Citation
Jc. Sutherland et al., Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis, ELECTROPHOR, 22(5), 2001, pp. 843-854
Citations number
40
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
01730835 → ACNP
Volume
22
Issue
5
Year of publication
2001
Pages
843 - 854
Database
ISI
SICI code
0173-0835(200103)22:5<843:QDDBGE>2.0.ZU;2-R
Abstract
DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quanti tative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge -coupled device (CCD) camera systems we developed to acquire images of fluo rophore labeled DNA.