Bronchoscopic bronchoalveolar lavage (BAL) may be followed by a systemic in
flammatory response, Previous reports have suggested pneumonia as a predisp
osing condition and systemic cytokines as possible mediators.
To test this hypothesis, systemic levels of interleukin (IL)-1 beta, IL-6 a
nd tumour necrosis factor-alpha (TNF-alpha) were studied before and at 12 h
and 24 h after bronchoscopically guided BAL in 30 mechanically ventilated
patients (median age 67 (range 54-76) yrs, simplified acute physiology scor
e TT (SAPS II) 33 (12-56)), 20 of whom had pneumonia and 10 of whom were co
ntrol patients without pneumonia, Arterial oxygen partial pressure to inspi
red oxygen fraction ratio (Pa2O2/FI,O-2), body temperature, mean arterial p
ressure, and cardiac frequency were recorded, The majority of patients (28/
30, 93%) received antibiotic treatment prior to the procedure.
Pa,O-2/FI,O-2 ratio was lower at 12 h compared to baseline in patients with
pneumonia (baseline median 192 (range 65-256); 12 h 160 (66-190) mmHg, p <
0.001) and ventilated controls (baseline 293 (205-473); 12 h 226 (153-330)
mm Hg p = 0.011), but returned to baseline levels at 24 h (pneumonia: 194
(92-312), p = 0.991; controls: 309 (173-487) mmHg, p = 0.785), No changes i
n other clinical variables were observed. Systemic TNF-<alpha> levels befor
e BAL (pneumonia: 35 (10-88); controls: 17 (0-33) pg.mL(-1)) did not increa
se at 12 h (pneumonia: 35 (0-64); p = 0.735; controls: 16 (0-21) pg.mL(-1),
p = 0.123 comparison to baseline) or 24 h (pneumonia: 31 (0-36), p = 0.464
; controls: 19 (0-43) pg.mL(-1), p = 0.358), No changes of IL-1 beta (basel
ine: pneumonia 0 (0-13); controls 1 (0-32) pg.mL(-1)) or IL-6 (baseline: pn
eumonia, 226 (9-4300); controls, 53 (0-346) pg.mL(-1)) were detected.
No deterioration of clinical variables and no increase in systemic cytokine
release has been observed after bronchoalveolar lavage, in critically ill
patients. The potential cytokine increase is probably too small, in relatio
n to the pre-existing inflammatory response, to yield clinical significance
in this population otherwise antibiotic therapy may have been protective.