Allele frequencies for human deoxyribonuclease I (DNase I) phenotypes were
determined using blood samples from about 2000 Japanese subjects living in
nine prefectures, and compared with one another. DNase I phenotyping was pe
rformed principally using isoelectric focusing electrophoresis and activity
staining. The DNase I system was shown to have enhanced potential for anth
ropologic, genetic, and clinical studies of Japanese populations. DNase I p
henotypes were analyzed to evaluate the degree of genetic variation at the
DNASE1 locus. Our examination of DNase I types revealed a decreasing north-
to-south gradient in the DNASE1 allele.