Renewable resources, such as rapeseed oil or oleic acid, combined with gluc
ose were used both in a feed-batch and two-stage continuous process for the
production of sophorolipids with the yeast Candida bombicola ATCC 22214. H
igh yields > 300 g 1(-1) and increased productivities of 57 g 1(-1) d(-1) (
feed-batch) and 76 g 1(-1) d(-1) (continuous mode), respectively, were obta
ined by using optimized cultivation conditions. The key-factors for enhance
d sophorolipid production are presented. The acidic sophorolipid was used f
or further modification after alkaline hydrolysis and purification. A novel
glucoselipid was synthesized by using the enzyme naringinase (EC 3.2.1.40)
. a (omega -1) hydroxyfatty acid, commercially not yet available and diffic
ult to prepare by organic synthesis, was released by acidic hydrolysis, pur
ified and is proposed as a precursor for plastics and flavours. (C) 2001 El
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