D. Pangallo et al., Preparation of a MIMIC internal control for the multiplex PCR for Listeriamonocytogenes detection, IND ALI, 40(400), 2001, pp. 152-154
A mimic internal control for monitoring the efficiency of the multiplex PCR
for Listeria monocytogenes detection was prepared by low-stringency PCR wi
th Yersinia bercovieri DNA. The mimic internal control was sequenced. When
added in the multiplex PCR, the mimic internal control was always amplified
without affecting the detection limit of PCR. The simple method described
can be used for the preparation of mimic internal controls for further PCR
protocols detecting pathogens.